physical: Building 16.03 // The Station Crescent // Oteha Rohe
tel: +64 9 4140800 ext 41585 // email: p.remigi(at)massey.ac.nz
I did my PhD at INRA Toulouse (France) in the Laboratory of Plant-Microbe Interactions (LIPM). I worked in the team of C. Boucher and S. Genin, under the supervision of N. Peeters, where I studied the function and evolution of a family of type-III effectors from the plant pathogenic bacterium Ralstonia solanacearum. Using a combination of bioinformatics and functional analyses, we were able to propose a scenario for the evolution and the conservation of this gene family, which is essential for R. solanacearum’s pathogenicity on several host plants.
I further developed my interests in bacterial evolution during my first post-doc in C. Masson and J. Batut group, still at LIPM. I was involved in the analysis of an experimental evolution project that aimed at evolving a plant pathogenic bacterium into a legume symbiont. My project focused on the analysis of the unusually high number of mutations found in the evolved strains.
Evolution of phenotypic heterogeneity
It is now well established that genetically identical bacteria grown in a given environment can display different phenotypes. Several examples of this phenomenon, called “phenotypic heterogeneity”, have been well described mechanistically but remain poorly studied from an evolutionary perspective. A previous evolution experiment conducted in the lab led to the emergence of bacterial strains that switch at high frequency between 2 distinct phenotypic states (Beaumont et al., 2009). Building on the subsequent genetic and phenotypic characterization of these strains (Gallie et al., in preparation) and in collaboration with Gayle Ferguson, I would like to explore how selection can further shape this switching behaviour.
Gallie J., E. Libby, F. Bertels, P. Remigi, C.B. Jendresen, G.C. Ferguson, N. Desprat, M.F. Buffing, U. Sauer, H.J.E. Beaumont, J. Martinussen, M. Kilstrup, and P.B. Rainey. (2015) Bistability in a metabolic network underpins the de novo evolution of colony switching in Pseudomonas fluorescens SBW25. PLOS Biology 13(3):e1002109
Remigi P, Capela D, Clerissi C, Tasse L, Bouchez O, Batut J, Cruveiller S, Rocha EP, Masson-Boivin C. (2014) Transient hypermutagenesis accelerates the evolution of legume endosymbionts following horizontal gene transfer. PLOS Biology 12(9):e1001942.
Marchetti M, Jauneau A, Capela D, Remigi P, Gris C, Batut J, Masson-Boivin C. (2014) Shaping bacterial symbiosis with legumes by experimental evolution. MPMI 27(9): 956-64.
Remigi P, Anisimova M, Guidot A, Genin S, Peeters N. (2011) Functional diversification of the GALA type III effector family contributes to Ralstonia solanacearum adaptation on different plant hosts. New Phytol. 192, 976-87.
Reeser PW, Sutton W, Hansen EM, Remigi P, Adams GC. (2011) Phytophthora species in forest streams in Oregon and Alaska. Mycologia 103, 22-35.
Remigi P, Faye A, Kane A, Deruaz M, Thioulouse J, Cissoko M, Prin Y, Galiana A, Dreyfus B, Duponnois R. (2008). The exotic legume tree species Acacia holosericea alters microbial soil functionalities and the structure of the arbuscular mycorrhizal community. Appl Environ Microbiol. 74, 1485-93.